| Component | Volume (μL) | Final |
|---|---|---|
| Washed resin from step 9A(v) | --- | --- |
| tRNA (10 μg/μL) | 1.5 | 0.75 μg/μL |
| SUPERase-In (40 U/μL) | 1.5 | 3 U/μL |
| ATP (100 mM) | 0.3 | 1.5 mM |
| siLuc guide strand RNA (1×106 CPM/μL) | 1 | 1×106 CPM |
| 10x RNAi buffer | 3 | 1x |
| Nuclease-free water | 12.7 | --- |
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