| Component | Volume (μL) | Final | |||
|---|---|---|---|---|---|
| Tube 1 | Tube 2 | Tube 3 | Tube 4 | ||
| Cytoplasmic extract | 300 | 300 | --- | --- | 300 μL |
| Nuclear extract | --- | --- | 300 | 300 | |
| siLuc duplex siRNA (1×106 CPM/μL) | 1 | 1 | 1 | 1 | 1×106 CPM |
| ATP (100 mM) | 3 | 3 | 3 | 3 | 1 mM |
| Phosphocreatine (1 M) | 3 | 3 | 3 | 3 | 10 mM |
| Creatine Kinase (4 U/μL) | 3 | 3 | 3 | 3 | 0.04 U/ μL |
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