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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Chin Sci Bull. 2013 Apr;58(10):1162–1168. doi: 10.1007/s11434-012-5634-9

Figure 4.

Figure 4

Endpoint fluorescence values for DNA standards subjected to Non-Instrumented Nucleic Acid Amplification with Assimilating Probe. Reactions were run at ambient temperatures of 8°C (blue circles), 22°C (green triangles), and 36°C (red squares), with standards classified as positive designated by filled symbols those classified as negative by open symbols. Pooled data fit a logistic curve (a=41400 RFU; b=−1.69; x0=122 fg; y0=14390 RFU; R2=0.92, RMSE=5314 RFU) resulting in a detection limit of 92 fg DNA (vertical solid line, equivalent to 18 copies of the S. enterica genome). A single genome copy equivalent (5.1 fg) is represented by the vertical dashed line.