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. Author manuscript; available in PMC: 2015 Mar 29.
Published in final edited form as: Nat Commun. 2014 Sep 29;5:4976. doi: 10.1038/ncomms5976

Figure 7. Superposition of right-handed and left-handed structural ensembles.

Figure 7

The 10 top-ranking models ensemble produced by the hybrid structure determination approach considering (a) a right-handed helix and (b) a left-handed helix. The basic tetramer formed by the symmetric subunits (i, orange), (i+5, light green), (i+6, dark green) and (i+11, pink) is presented and N- and C- termini are indicated. The relative position of protein subunits is explained schematically in the top-left corner of (a) and (b) for the two handedness arrangements. In a right-handed helix, the subunit (i+5), closer to (i) in terms of axial translation, is located to the left of (i) while subunit (i+6), further away from (i) in terms of axial translation, is located to the right of it. Blue lines connect successive subunits as in Fig. 6. The right-handed ensemble shows much higher convergence compared to the left-handed ensemble. The right-handed ensemble presents less NMR constraint violations and has a more favorable full-atom energy, unequivocally identifying it as the correct handedness.