Table 1. Number of amino acids that gain alpha-helicity upon cooling from 37°C to −10°C, using different algorithms.

	Helix-Coil Model	SELCON3	CONTIN
Q25	23	21	23
Q46	31	33	30

Estimation of temperature-dependent structure of thioredoxin exon 1 HTT proteins. A. Number of amino acids in HTT exon 1 of each Q-length that experienced a temperature-dependent change in alpha-helical structure, as indicated, calculated either by the helix-coil model or using DICHROWEB software. Taking the varying results together, it is estimated that between 21 and 27 amino acids in the Q25 construct and between 30 and 37 amino acids in the Q46 construct gain alpha-helical structure upon cooling from 37°C to –10°C.