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. 2014 Nov 18;12:50. doi: 10.1186/s12953-014-0050-5

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© Klein-Scory et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Release of different amounts of CD73/NT5E proteins: a) NT5E/CD73 was detected in EV samples of pancreatic carcinoma cell lines in different amounts. No NT5E/CD73 was found in the samples of the normal model cells HPDE. The corresponding silver stained gel part is shown for the control. b) Ascites derived from PDAC and CRC (colorectal carcinoma) patients and the conditioned medium (CM) of primary cancer cells (isolated from ascites) were used to prepare the EV samples. Samples were tested for NT5E/CD73 by western blot. Additionally, samples of the CRC patients were checked. c) Human pancreatic stellate cells (HPSC) also release some of the EV proteins, but in a lesser amount than the pancreatic cancer cell line. An example of corresponding silver stained gel is given. Ly, lysate; Secr, secretome; EV, EV crude preparation.