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. 2014 Nov 25;14:198. doi: 10.1186/s12876-014-0198-6

Table 2.

Primer sequences for quantitative PCR

Gene Forward/reverse Primer sequence
GAPDH Forward AGCCCAGAACATCATCCCTG
Reverse CACCACCTTCTTGATGTCATC
TGF-β1 Forward CCTGGAAAGGGCTCAACAC
Reverse CAGTTCTTCTCTGTGGAGCTGA
Collagen-1 Forward CATGTTCAGCTTTGTGGACCT
Reverse GCAGCTGACTTCAGGGATGT
α-SMA Forward CGATAGAACACGGCATCATCAC
Reverse GCATAGCCCTCATAGATAGGCA

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TGF-β1, transforming growth factor-beta 1; collagen-1, type 1 collagen; α-SMA, α-smooth muscle actin.