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. 2014 Dec 2;9(12):e110534. doi: 10.1371/journal.pone.0110534

Figure 2. a–d: Light micrographs of the hippocampal CA1 neurons stained with anti-saposin D (a, b) or anti-PS IgG (c, d) showing the PS-IR 3 days after 5 mg/kg KA injection.

Figure 2

The dendritic structure of the CA1 region was stained green with an anti-Map II antibody (a, c). b and d show higher-magnification images of the pyramidal layer (Pyr) shown in a and c, respectively. The arrow indicates an interneuron with a slender nucleus and very intense PS-IR cytoplasm (d). e-h: Light micrographs of the hippocampal CA1 neurons of normal controls (e) and animals 3 days after 5 mg/kg KA injection (g). f and h are black-and-white images of the pyramidal layer (Pyr) shown in figures e and g, respectively, which were used for the NIH Image analysis. (i, j) Bar  = 100 µm (a, c) and 200 µm (b, d–h). i, j: The ratio of PS-IR in the hippocampal CA1 region after injection of KA or saline. PS-IR was semi-quantified using NIH image analysis. The ratio of PS-IR was significantly higher on days 1, 3, 7, 14 and 21 after KA injection compared with controls injected with saline (* p<0.05, ** p<0.01). Mol, stratum moleculare; Rad, stratum radiatum; Ori, stratum orience.