Figure 5. Knockdown of BMPRII did not affect BMP4 elevated basal [Ca²⁺]_i and SOCE in PASMCs.

(A) bar graph showing the changes in basal [Ca²⁺]_i in distal PASMCs treated with 50 ng/ml BMP4 (n  =  4 from 120 cells) for 60 h. Bar values are means ± SEM. *P <0.05 vs. siNT control (n  =  4 from 120 cells). (B) represting time course of quenching of Fura-2 AM fluorescence at 360 nm by 200 µM Mn²⁺ after perfusion with Ca²⁺ -free KRBS (0 Ca²⁺) containing Nifedipine (5 µM) and CPA (10 µM) in distal PASMCs treated with 50 ng/ml BMP4 (n = 4 experiments in 110 cells) or vehicle for 60 h, normalized to fluorescence at time 0 (F/F₀). (C) Bar graph shows that Mn²⁺ quenching, expressed as percent decrease in fluorescence at time 10 min from time 0, was greater in BMP4-treated distal PASMCs compared with that in control cells (*P<0.05). Bar values are means ± SEM.