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. 2014 Nov 25;11:99. doi: 10.1186/s12977-014-0099-3

Figure 7.

Figure 7

Coreceptor usage of mutants targeting the tip of V1 region and the base of V2 loop. Recombinant viruses with mutations targeting the tip of V1 region (MJC97mtV1 and MJC97mtV1′) or with additional mutations in the base of V2 loop (MJC97mtV1V2 and MJC97mtV1V2′). The details of these two sets of mutants are described in Figure 6. Viral replication was followed-up for 12 days by assessing RT activity in culture supernatants of infected cells. The highest value of RT activity observed during this time period was used. Results are expressed as the mean of three independent experiments performed in duplicate. Error bars represent the standard error of the mean. A star (*) indicates statistical significant difference (p < 0.001) between the means of peak RT activity measured in culture supernatants of GHOST-CD4/CCR8, GHOST-CD4/CCR5 and GHOST-CD4/CXCR4 inoculated with MJC97mt6 and MJC97mtV1 or MJC97mt7 and MJC97mtV1V2. A double star (**) indicates statistical significant difference (p < 0.001) between the means of peak RT activity measured in culture supernatants of GHOST-CD4/CCR8 and GHOST-CD4/CCR5 inoculated with MJC97mt6′ and MJC97mtV1′ or MJC97mt7′ and MJC97mtV1V2′.