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. 2014 Nov 25;11:100. doi: 10.1186/s12977-014-0100-1

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© Slaughter et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DLS analysis of BI-D induced oligomerization of recombinant WT and the H171T INs. Shown are the size distributions (%) of IN after DMSO treatment (blue) or BI-D treatment after 15 minutes (red), 20 minutes (green) and 30 minutes (yellow) incubation. BI-D treatments include (A) WT IN +0.120 μM BI-D, (B) H171T IN +0.120 μM BI-D, (C) WT IN +10 μM BI-D and (D) H171T IN +10 μM BI-D. The peak with the diameter size of <1 nm detected in these samples has also been observed for the buffer alone sample indicating that small size particles unrelated to IN or BI-D were present in our preparations.