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. 2014 Nov 25;11:199. doi: 10.1186/s12985-014-0199-7

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© Ben Abdeljelil et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Amino acid sequence alignment of the polyprotein sequences of the Tunisian vvIBDV wild-type PO7 strain (PO7wt), the cell culture-adapted and attenuated P2 and OKYMT IBDV strains, and the cell culture-adapted, but virulent, D279N/A284T mutated P2/PO7 chimeric IBDV virus (P2/PO7mt). Amino acid residues (positions 253, 279, and 284) known to be involved in cell culture adaptation and virulence are highlighted in green. The residue 256 which could have contributed to OKYMT attenuation is highlighted in orange.