Table 1.
Primers used in PCR amplifications, cloning and mutagenesis of IBDV segments A and B
| Primer designation | Sequence (5′-3′) | Orientation | Location | Restriction site |
|---|---|---|---|---|
| IB2SP1 | CATGGTACCATGACAAACCTGCAAGATCAAACC | + | 131-154 | KpnI |
| SAR1 | AGAGAATTCAGGGGACCCGCGAACGGATCCAATT | - | 3237-3261 | EcoRI |
| MutS | GCAAACAATGGGCTAACGACCGGCACTGACAACC | - | 962-995 | - |
| MutR | GGTCGTTAGCCCATTGTTTGCGGCCACAGCTCTG | - | 949-982 | - |
| SAR2 | CAAGAATCCCGTCGACTACG | - | 1739-1720 | SalI |
| SAS1 | GGATACGATCGGTCTGACCC | + | 1-20 | - |
| SBS1 | GGATACGATGGGTCTGACCC | + | 1-20 | - |
| SBR1 | GGGGCCCCCGCAGGCGAAGG | - | 2826-2807 | - |
Restriction enzyme sites are italicized.
Nucleotides used for mutagenesis of VP2 residues 279 and 284 are indicated in bold.