Table 1.
Bacterial strains and plasmids used in this study
| Strains and plasmids | Relevant features | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli strains | ||
| E.coli EC101 | Cloning host; repA + kmr | [37] |
| E.coli EC101-pNZ-M.Bbrll + Bbr11 | EC101 harboring a pNZ8048 derivative containing bbrllM and bbrlllM | [39] |
| Bifidobacterium sp. strains | ||
| B. breve UCC2003 | Isolate from a nursling stool | [36] |
| B. breve UCC2003 - pAM5 | B. breve UCC2003 harboring pAM5 | This study |
| B. breve UCC2003-nanA | pORI19-Tet-nanA (Bbr_0168) insertion mutant of B. breve UCC2003 | [13] |
| B. breve UCC2003-fucP | pORI19-Tet-fucP (Bbr_1742) insertion mutant of B. breve UCC2003 | This study |
| B. breve UCC2003-lnbP | pORI19-Tet-lnbP (Bbr_1587) insertion mutant of B. breve UCC2003 | This study |
| B. breve UCC2003-lacZ7 | pORI19-Tet-lacZ7 (Bbr_1833) insertion mutant of B. breve UCC2003 | This study |
| B. breve UCC2003-galT | Tetr transposon mutant of B. breve UCC2003 | [41] |
| B. bifidum PRL2010 | Isolate from infant faeces | [26] |
| B. bifidum PRL2010-pPKCM7 | B. bifidum PRL2010 harboring pPKCM7 | This study |
| Plasmids | ||
| pAM5 | pBC1-puC19-Tcr | [38] |
| pPKCM7 | pblueCm harboring rep pCIBA089 | [40] |
| pORI19 | Emr, repA−, ori+, cloning vector | [37] |
| pORI19-tetW-fucP | Internal 400 bp fragment of fucP and tetW cloned in pORI19 | This study |
| pORI19-tetW-lnbP | Internal 479 bp fragment of lnbP and tetW cloned in pORI19 | This study |
| pORI19-tetW-lacZ7 | Internal 568 bp fragment of lacZ7 and tetW cloned in pORI19 | This study |