Table 2.
Oligonucleotide primers used in this study
| Purpose | Primer | Sequence |
|---|---|---|
| Cloning of 400 bp fragment of fucP (Bbr_1742) in pORI19 | FucPF | TAGCATAAGCTTGGCGAATCGTTCGTATCA |
| FucPR | GATATCTCTAGAGCGCCCCAGTGCTTGAGC | |
| Cloning of 479 bp fragment of lnbP (Bbr_1587) in pORI19 | LnbPF | TAGCATAAGCTTCACACAGGTATTGGGAGGTTG |
| LnbPR | CTAGTCTCTAGAGTTGTAGGCGCCACCATCC | |
| Cloning of 568 bp fragment of lacZ7 (Bbr_1833) in pORI19 | LacZ7F | TAGCATAAGCTTCCAGGCCAAGAACTCCAGTG |
| LacZ7R | CATGATTCTAGACAGCTTGGGCAGGTTGAACG | |
| Amplification of tetW | TetWF | TCAGCTGTCGACATGCTCATGTACGGTAAG |
| TetWR | GCGACGGTCGACCATTACCTTCTGAAACATA | |
| Confirmation of site-specific homologous recombination | FucPconfirm | TGTTCGCCATGTTCGTTATC |
| LnbPconfirm | GATCACTCTGCATATGGACG | |
| LacZ7confirm | GTACCGACATCGACGCGTTC |
Restriction sites incorporated into oligonucleotide primer sequences are indicated in italics.