Table 3.
Effect of mucin (in co-culture with B. bifidum PRL2010) on the transcriptome of B. breve UCC2003
| Gene No. | Predicted Function | Level of upregulation ( P < 0.001) |
|---|---|---|
| Bbr_0161_ nanK | Conserved hypothetical protein in ROK family | 4.22 |
| Bbr_0162_ nanE | N-acetylmannosamine-6-phosphate 2-epimerase | 5.46 |
| Bbr_0164_ nanB | ABC transport system, solute binding protein | 14.08 |
| Bbr_0165_ nanC | ABC transport system, permease protein | 11.82 |
| Bbr_0166_ nanD | ABC transport system, ATP-binding protein | 18.92 |
| Bbr_0167_ nanF | ABC transport system, ATP-binding protein | 22.14 |
| Bbr_0168_ nanA | N-acetylneuraminate lyase | 15.17 |
| Bbr_0169_ nagB1 | Glucosamine-6-phosphate isomerase | 17.77 |
| Bbr_0171_ nanH | Sialidase | 7.23 |
| Bbr_0173_ nanR | Transcriptional regulator, GntR family | 4.80 |
| Bbr_1247_ nagA2 | N-acetylglucosamine-6-phosphate deacetylase | 4.59 |
| Bbr_1248_ nagB3 | Glucosamine-6-phosphate isomerase | 6.08 |
| Bbr_1585_ galE | UDP-Glc-4-epimerase | 3.96 |
| Bbr_1586_ nahK | N-acetylhexosamine kinase | 3.67 |
| Bbr_1587_ lnbP | Lacto-N-biose phosphorylase | 3.19 |
| Bbr_1588 | ABC transport system, permease protein | 3.34 |
| Bbr_1589 | ABC transport system, permease protein | 2.88 |
| Bbr_1590 | ABC transport system, solute binding protein | 8.95 |
| Bbr_1740_ dapA4 | Dihydrodipicolinate synthase | 9.91 |
| Bbr_1741 | Conserved hypothetical protein | 8.83 |
| Bbr_1742_ fucP | L-fucose permease | 7.81 |
| Bbr_1833_ lacZ7 | Beta-galactosidase | 4.31 |
| Bbr_1879 | PTS system, glucose-specific IIABC component | 6.97 |
| Bbr_1880 | PTS system, GlcNAc-specific IIBC component | 18.15 |
| Bbr_1884_ galT2 | Gal-1-phosphate uridylyltransferase | 3.86 |
The cut-off point for the level of up-regulation is 2.5-fold with a P-value of <0.001.