Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 12;9:179. doi: 10.1186/1748-717X-9-179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Wu et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Down-regulation of BTG1 significantly increases cellular radiation sensitivity in 786-O cells. (A) The down-regulation of endogenous BTG1 protein following transfection with siRNA oligonucleotides against BTG1 (siRNA-BTG1) or nonsense small RNA oligonucleotides (negative control, NC) were compared to untransfected (blank) cells. GAPDH serves as loading control. Fold changes of protein levels represent the average of three separate blots. (B) The percentage of 786-O cell viability after transfection with siRNA oligonucleotides against BTG1 (siRNA-BTG1) or nonsense small RNA oligonucleotides (negative control, NC) was determined by trypan blue dye exclusion assay at 48 h after X-ray exposure. (C) Effects of nonsense small RNA oligonucleotides or siRNA-BTG1 over-expression on caspase-3 activity in 786-O cells without irradiation or with 5 Gy of X-rays. (D) Colony formation assay of 786-O cells transfected with NC or siRNA-BTG1. (E) Representative plates of survival fraction of 786-O cells after various treatments. The data (means ± SE) are representative for at least five independent experiments.