Skip to main content
. Author manuscript; available in PMC: 2015 Nov 3.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2014 Nov 3;31:1C.17.1–1C.17.13. doi: 10.1002/9780470151808.sc01c17s31

Figure 5.

Figure 5

Important Factors affecting cryopreservation efficiency. The cryopreservation efficiency was calculated by using cell survival after cryopreservation normalized by the survival without cryopreservation. A. Dissociation method is critical for success of cryopreservation. H1 ESCs were dissociated by Dispase or EDTA/PBS, frozen in 10% DMSO/EDTA, and then recovered in E8. The cell survival was analyzed after 24 hours. B. DMSO dosage is important for cryopreservation. H1 ESCs were dissociated by EDTA/PBS, frozen in different concentration of DMSO in E8, and then recovered in E8. The cell survival was analyzed after 24 hours. C. Protein content is not critical for efficiency of cryopreservation. H1 ESCs were dissociated by EDTA/PBS, frozen in Base Medium DMEM/F12 with or without 1% Bovine Serum Albumin (BSA), and then recovered in E8. The cell survival was analyzed after 24 hours.