. 2014 Dec 2;9(12):e113796. doi: 10.1371/journal.pone.0113796

Table 1. Bacterial strains and plasmids.

Name	Relevant Characteristics	Comments/Source/Reference
Strains
Streptococcus pneumoniae
R36A	Non-encapsulated laboratory strain.	[20]
BCSJC001	R36ApBCSJC001, Tet^r.	[3]
BCSJC006	R36ApBCSJC006, Tet^r.	[3]
BCSJC011	R36ApBCSJC011, Tet^r.	This work.
BCSJC012	R36ApBCSJC012, Tet^r.	This work.
BCSJC013	R36ApBCSJC013, Tet^r.	This work.
BCSJC014	R36ApBCSJC014, Tet^r.	This work.
BCSJC015	R36ApBCSJC015, Tet^r.	This work.
BCSJC016	R36ApBCSJC016, Tet^r.	This work.
BCSJC017	R36ApBCSJC017, Tet^r.	This work.
BCSJC018	R36ApBCSJC018, Tet^r.	This work.
BCSJC019	R36ApBCSJC019, Tet^r.	This work.
BCSJC020	R36ApBCSJC020, Tet^r.	This work.
BCSJC021	R36ApBCSJC021, Tet^r.	This work.
BCSJC022	R36ApBCSJC022, Tet^r.	This work.
BCSJC023	R36ApBCSJC023, Tet^r.	This work.
BCSJC024	R36ApBCSJC024, Tet^r.	This work.
BCSJC025	R36ApBCSJC025, Tet^r.	This work.
BCSJC026	R36ApBCSJC026, Tet^r.	This work.
BCSJC027	R36ApBCSJC027, Tet^r.	This work.
BCSJC028	R36ApBCSJC028, Tet^r.	This work.
BCSJC029	R36ApBCSJC029, Tet^r.	This work.
BCSJC030	R36ApBCSJC030, Tet^r.	This work.
BCSJC031	R36ApBCSJC031, Tet^r.	This work.
BCSJC032	R36ApBCSJC032, Tet^r.	This work.
BCSJC033	R36ApBCSJC033, Tet^r.	This work.
BCSJC034	R36ApBCSJC034, Tet^r.	This work.
BCSJC035	R36ApBCSJC035, Tet^r.	This work.
BCSJC036	R36ApBCSJC036, Tet^r.	This work.
BCSJC037	R36ApBCSJC037, Tet^r.	This work.
BCSJC038	R36ApBCSJC038, Tet^r.	This work.
BCSJC047	R36ApBCSJC043, Tet^r.	This work.
BCSMH008	R36ApBCSMH007, Tet^r.	[3]
BCSMH030	R36ApBCSLF001, Tet^r.	[3]
BCSMH033	R36ApBCSMH002, Tet^r.	[3]
BCSMH063	R36ApBCSMH061, Tet^r.	This work.
Lactococcus lactis
LL108	RepA⁺ MG1363, Cm^r	[21]
BCSJC039	LL108pBCSMH002, Tet^r.	This work.
BCSJC040	LL108pBCSJC001, Tet^r.	This work.
Staphylococcus aureus
RN4220	Restriction deficient derivative of S. aureus NCTC8325-4 strain capable of receiving foreign DNA	R. Novick
BCSJC041	RN4220pBCSJC039, Amp^r; Ery^r; LacZ⁺	This work.
BCSJC042	RN4220pBCSJC040, Amp^r; Ery^r; LacZ⁺	This work.
Bacillus subtilis
MB24	trpC2 metC3	Laboratory stock
BCSJC043	MB24pBCSJC039, Amp^r; Ery^r; LacZ⁺	This work.
BCSJC044	MB24pBCSJC040, Amp^r; Ery^r; LacZ⁺	This work.
S. pneumoniae plasmids
pBCSJC001	pBCSMH004 derivative, allowing expression of Citrine containing the first 10 aa of Wze at its N-terminus, Tet^r.	[3]
pBCSJC006	pBCSJC001 derivative, TTA→CTC change in codon 4 of Wze, Tet^r.	[3]
pBCSJC011	pBCSMH007 derivative, allowing expression of Citrine containing the first 10 aa of Wzd at its N-terminus, Tet^r	This work.
pBCSJC012	pBCSJC037 derivative, allowing expression of Citrine containing the first 12 aa of MurM at its N-terminus, Tet^r	This work.
pBCSJC013	pBCSJC038 derivative, allowing expression of Citrine containing the first 10 aa of MurN at its N-terminus, Tet^r	This work.
pBCSJC014	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MLEPTIAQKKL at its N-terminus, Tet^r	This work.
pBCSJC015	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPLETIAQKKL at its N-terminus, Tet^r	This work.
pBCSJC016	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPTILEAQKKL at its N-terminus, Tet^r	This work.
pBCSJC017	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPTIALEQKKL at its N-terminus, Tet^r	This work.
pBCSJC018	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPTIAQLEKKL at its N-terminus, Tet^r	This work.
pBCSJC019	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPTIAQKLEKL at its N-terminus, Tet^r	This work.
pBCSJC020	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPTIAQKKLEL at its N-terminus, Tet^r	This work.
pBCSJC021	pBCSJC001 derivative, allowing expression of Citrine containing the sequence MPTIAQKKLLE at its N-terminus, Tet^r	This work.
pBCSJC022	pBCSJC001 derivative, TTA→TTG change in codon 4 of Wze, Tet^r.	This work.
pBCSJC023	pBCSJC001 derivative, TTA→CTT change in codon 4 of Wze, Tet^r.	This work.
pBCSJC024	pBCSJC001 derivative, TTA→CTA change in codon 4 of Wze, Tet^r.	This work.
pBCSJC025	pBCSJC001 derivative, TTA→CTG change in codon 4 of Wze, Tet^r.	This work.
pBCSJC026	pBCSJC001 derivative, GAA→GAG change in codon 5 of Wze, Tet^r.	This work.
pBCSJC027	pBCSJC001 derivative, TTA→TTG change in codon 4 of Wze and GAA→GAG change in codon 5 of Wze, Tet^r.	This work.
pBCSJC028	pBCSJC001 derivative, TTA→CTC change in codon 4 of Wze and GAA→GAG change in codon 5 of Wze, Tet^r.	This work.
pBCSJC029	pBCSJC001 derivative, TTA→CTA change in codon 4 of Wze and GAA→GAG change in codon 5 of Wze, Tet^r.	This work.
pBCSJC030	pBCSJC001 derivative, TTA→CTT change in codon 4 of Wze and GAA→GAG change in codon 5 of Wze, Tet^r.	This work.
pBCSJC031	pBCSJC001 derivative, TTA→CTG change in codon 4 of Wze and GAA→GAG change in codon 5 of Wze, Tet^r.	This work.
pBCSJC032	pBCSJC013 derivative CTC→CTG change in codon 6 of MurN, Tet^r.	This work.
pBCSJC033	pBCSJC013 derivative CTC→CTT change in codon 6 of MurN, Tet^r.	This work.
pBCSJC034	pBCSJC013 derivative CTC→CTA change in codon 6 of MurN, Tet^r.	This work.
pBCSJC035	pBCSJC013 derivative CTC→TTG change in codon 6 of MurN, Tet^r.	This work.
pBCSJC036	pBCSJC013 derivative CTC→TTA change in codon 6 of MurN, Tet^r.	This work.
pBCSJC037	pBCSMH002 containing murM-Citrine, Tet^r.	This work.
pBCSJC038	pBCSMH002 containing murN-Citrine, Tet^r.	This work.
pBCSJC043	pBCSJC001 derivative, TTA→CTC change in codon 4 of Wze, GAA→GAG change in codon 5 of Wze and ATA→AGA change in codon 6 of Wze, Tet^r.	This work.
pBCSLF001	High-copy-number vector, contains the -10 constitutive promoter of SigA from S. pneumoniae, Tet^r.	[4]
pBCSMH002	pBCSLF001 derivative, allows expression of Citrine fusion proteins, Tet^r.	[4]
pBCSMH007	pBCSMH002 containing wzd-Citrine, Tet^r.	[4]
pBCSMH060	pBCSMH002 containing wchA-Citrine, Tet^r.	This work.
pBCSMH061	pBCSMH060 derivative allowing expression of Citrine containing the first 11 aa of WchA at its N-terminus, Tet^r	This work.
S. aureus/B. subtilis plasmids
pMAD	E. coli – S. aureus shuttle vector with a thermosensitive origin of replication for Gram-positive bacteria; Amp^r; Ery^r; LacZ⁺	[7]
pBCSJC039	pMAD derivative containing the -10 constitutive promoter of SigA from S. pneumoniae, the ribosome-binding site and citrine, Amp^r; Ery^r; LacZ⁺	This work.
pBCSJC040	pMAD derivative containing the -10 constitutive promoter of SigA from S. pneumoniae, the ribosome-binding site and icitrine, Amp^r; Ery^r; LacZ⁺	This work.