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. 2014 Dec 2;9(12):e113740. doi: 10.1371/journal.pone.0113740

Figure 1. Confirmation of a cloned hcmv-miRUL112-3p TaqMan PCR amplicon from in vitro infected HCMV lung fibroblasts (Positive Control): Alignment of sequences (from above) shows the PCR2.1 TOPO empty plasmid, hcmv-miR-UL112 sequence from miRBase database and last two sequences confirms the PCR product was inserted into the PCR2.1 TOPO plasmid.

Figure 1

The lower part represents the chromatogram for the recombinant plasmid.