Table 1. Operational taxonomic units (OTUs) present at >1% of total sequence reads in supra-gingival plaque isolated 24 hour and 48 hours post de-scale and polish.

OTU	Species	Percentidentity	Percentcoverage	Total number ofsequence reads	Percentage of totalsequence reads
2184	Bergeyella zoohelcum	99.14	100	29467	16.97
637	Neisseria shayeganii	98.57	100	18068	10.41
683	Moraxella sp. COT-017	100	100	16167	9.31
991	Pasteurellaceae bacterium COT-080	100	100	11742	6.76
2629	Porphyromonas cangingivalis	99.71	100	9746	5.61
613	Neisseria weaveri	100	100	7862	4.53
2795	Capnocytophaga sp. COT-339	100	100	4951	2.85
163	Fusobacterium sp. COT-189	99.14	100	4096	2.36
2083	Pasteurella dagmatis	100	100	3447	1.99
580*	Actinomyces sp.	100	100	2902	1.67
1122	Lautropia sp. COT-175	99.71	100	2842	1.64
1366	Capnocytophaga cynodegmi	100	100	2593	1.49
326	Neisseria zoodegmatis	99.71	100	2588	1.49
21*	Corynebacterium sp.	100	100	2526	1.45
2160	Stenotrophomonas sp. COT-224	98.58	100	1960	1.13
234	Globicatella sp. COT-107	100	100	1948	1.12
226	Capnocytophaga canimorsus	100	100	1768	1.02

Cut-offs of 98.5% were applied for identity and coverage (alignment length divided by query length) for annotation to species level, if the alignment did not meet the cut-off criteria the best hit from the Silva database was chosen (indicated by *).