Figure 7. Automated quantification of L and S cones in predetermined-fixed-size circular areas of the retina.

Top row. Composite micrographs of predetermined fixed-size circular areas (PCA) from one animal with LIP. Immunolabeled L- and S-cone outer segments in PCA from a representative left (light exposed) (same retina illustrated in Fig. 5B,B′) and right retina, 7 days after LIP. For each experimental retina and also within the corresponding region of the right-fellow retina, the numbers of L- or S-cones were counted within PCA of 3.14 mm² or 5.3 mm², respectively, superimposed upon the centre of the lesion. Bottom row. A custom-written macro allowed representation of every detected cone outer segment by a white dot within the PCA. Total numbers of L- or S-cones in these PCA was 7,118±842 or 661±125 for the light exposed retinas and 14,040±1,860 or 2,255±193 for control retinas, respectively (n = 7). Bar: 1 mm.