Table 1. Quantitative analysis of LIP retinal damage.

	Total number of cones in retinal wholemounts				Cones in predetermined fixed-size circular areas (PCA)				Area of retina		Area of damaged)		Diameter length of damage (µm)
Rat	L-cones		S-cones		L-cones		S-cones		(mm2)		region (mm2		Measured on retinal wholemounts			Measured
	Right retina	Left retina	Right retina	Left retina	Right retina	Left retina	Right retina	Left retina	Right retina	Left retina	L-cones	S-cones	L-cones	S-cones	average	by OCT
C	215,894	200,429	34,892	35,707	14,521	7,025	2,556	625	58.2	55.0	0.57	2.99	952	1,882	1,417	1,390
D	219,235	223,050	41,258	35,057	17,001	7,753	1,998	496	58.3	57.6	0.76	3.92	892	2,105	1,499	1,443
E	206,983	215,943	37,489	28,757	13,986	6,136	2,204	729	56.2	57.2	0.86	3.69	986	2,039	1,513	1,404
F	236,874	193,050	30,980	36,994	11,587	5,897	2,369	642	56.6	58.6	0.94	3.18	1,107	1,701	1,404	1,319
G	238,025	210,199	39,568	29,876	14,789	7,249	2,179	894	58.0	59.2	0.60	3.71	774	2,114	1,444	1,373
H	228,654	250,188	41,274	34,340	14,568	7,568	2,089	655	53.5	54.6	0.51	4.03	774	2,381	1,578	1,467
I	211,089	208,956	40,153	36,029	11,827	8,196	2,391	584	57.6	55.4	0.53	3.46	773	2,270	1,521	1,458
mean	222,393 *	214,545 *	37,945 †	33,823 †	14,040 ‡	7,118 ‡	2,255 §	661 §	56.9 #	56.8 #	0.68	3.57	894	2,070	1,482	1,408
SD	12,320	18,512	3,821	3,202	1,860	842	193	125	1.7	1.8	0.17	0.38	130	228	63	53

Total numbers of cones automatically quantified in retinal wholemounts from experimental rats whose left eye had been exposed to Light Emitting Diode-induced phototoxicity (LIP) and were analysed seven days later. The numbers of cones within predetermined fixed-size circular areas (PCA) were also automatically quantified. The total areas of the retinas (Area of retina) as well as the areas lacking L- or S-cones (Area of damaged region) were measured over flat-mounted retinas. The maximum diameters (Diameter length of damage) of the areas lacking L- or S-cones were measured over flat-mounted retinas and also the length of ONL lacking cell nuclei was measured in vivo with OCT. Statistical analysis: *T-test p = 0.369; †T-test p = 0.049; ‡T-test p≤0.001; §T-test p≤0.001; # When compared with the areas of naïve retinas (see Table 2) there were no significant differences (ANOVA p = 0.974).