Figure 3. Recombinant F-5 fragment of Hsp90α promoted wound healing in normal pigs.

(A) Picture of a typical 25–30 lb and healthy farm pig used in experiments. (B) Wounds (2.0 cm×2.0 cm) in triplicates were topically treated once on day 0 with either CMC gel alone or CMC gel containing recombinant F-5 protein (45 µM). Wounds in triplicate were photographed on the days indicated and analyzed for wound closure rates. (C) Quantitative analyses of the wound closure were presented. (D) Wedge biopsies were made on day 14 wounds, sectioned and stained with H&E. This experiment was repeated four times (a single surgery conducted in four separate pigs) and the results were reproducible. * p≤0.05, ** p≤0.01 and *** p≤0.001, compared with the placebo.