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. 2014 Dec 2;9(12):e113867. doi: 10.1371/journal.pone.0113867

Figure 3. Inhibition of XPO1 by siRNA transfection increased nuclear p21 in RCC cells.

Figure 3

The RCC (786-O and ACHN) cells were grown to ∼60% confluence and were transfected with scrambled sequence control or XPO1-specific siRNA for 48 hours. Immunoblotting was accomplished with specific antibodies (A) and immunofluorescence was performed by confocal microscopy (20x) (B). The number of cells in which p21 was predominantly in the nucleus were counted in three randomly selected fields and divided by the total cell number. Blue, nucleus (DAPI); Green, p21. *P<0.05 compared to control. Error bars indicate standard deviation.