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. 2014 Dec 2;9(12):e114196. doi: 10.1371/journal.pone.0114196

Figure 6. Effects of indole-3-acetic acid produced by Ustilago esculenta strain JYC070 on auxin-regulated gene expression.

Figure 6

GUS staining of DR5::uidA Arabidopsis seedlings grown in the supernatants of 5-d yeast cultures. (A–C) Control group inoculated in YPD medium containing L-tryptophan. In untreated control plants, DR5::uidA was absent from hypocotyls and young leaves, and was expressed primarily in the leaf margin. (D–F) Seedlings grown in medium supplied with 10 µg/mL IAA produced by U. esculenta. (G–I) Seedlings grown in medium supplemented with 100 µg/mL IAA produced by U. esculenta. We observed upregulated GUS expression in hypocotyls and young leaves following IAA treatment. Results are obtained from duplicate experiments with similar results. When we treated the transgenic seedlings with the supernatants of other yeasts, we obtained similar results.