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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Neuroimmunol. 2014 Sep 16;276(0):142–149. doi: 10.1016/j.jneuroim.2014.09.006

Figure 4.

Figure 4

Cytokine responses during the early stage of EAE. MNCs from RORγt Tg mice (open bars) produced significantly interleukin (IL)-17 (A) and IL-10 (B) IL-6 (C) and interferon (IFN)-γ (D) in response to MOG compared with control mice (closed bars) (*, P < 0.05, t test). Concanavalin A (ConA) also induced higher IL-17, IL-10 and IL-6 but not IFN-γ production in RORγt Tg mice than wild-type mice. Mononuclear cells (MNCs) were isolated from the inguinal lymph nodes of RORγt or wild-type mice (19 days p.i.) and stimulated with ConA or MOG35-55 for 48 hours (hr). The levels of cytokine production in the culture supernatants were measured by ELISA. The results are representative of two independent experiments consisting of 9 or 11 mice, for a total of 9 wild-type and 11 RORγt Tg mice. Results are mean + SEM from three pools of inguinal lymph nodes from two mice per pool. ND: Not detectable.