Table 1.
Reagents and solutions used in DNA isolation.
| List of reagents used | Solution preparationa | |
|---|---|---|
| Glycine (Merck Catalogue# 3570) Tris (Qualigens Catalogue# 10645) EDTA (Qualigens Catalogue# 118445000) Phenol (Sisco Research Laboratories Catalogue# 1649142) Chloroform (Qualigens Catalogue# 12307) Isoamyl alcohol (Sisco Research Laboratories Catalogue# 092945) 100% absolute Alcohol (Merck Emsure Catalogue# 100983) SDS (Life Technologies Catalog# 15525017) Proteinase K (Sigma–Aldrich Catalog#P2308) |
1X GTE buffer: | 100 mM glycine 10 mM Tris-HCL pH 8.0 1 mM EDTA |
| Cell Lysis Solution: | 10 mM Tris–HCl pH 8.0 0.1 mM EDTA pH 8.0 0.5% SDS |
|
| 24:1 v/v Chloroform and Isoamyl alcohol | ||
| 10 mg of Proteinase K in 1 ml of MiliQ (10 mg/ml) | ||
a
HINT: Reconstitute for the first time, aliquot it in 250 ul tubes, for uses, then store at −20 °C and reuse.