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. 2014 Aug 30;4(2):109–113. doi: 10.1016/j.jobcr.2014.08.004

Table 2.

Details of PCR primers & conditions.

Primer Sequence Amplification conditions
Forward 5′ ACAATTGAATGTCTGCACAGCCACTT 3′ 94 °C for 5 min, 35 cycles of 94 °C for 45 s, 58 °C for 45 s and 72 °C for 45 s, followed by 72 °C for 10 min
Reverse 5′ GGCAGAGATGTGTTTAAGTGCTG 3′