Table 2.
Primer | Sequence | Amplification conditions |
---|---|---|
Forward | 5′ ACAATTGAATGTCTGCACAGCCACTT 3′ | 94 °C for 5 min, 35 cycles of 94 °C for 45 s, 58 °C for 45 s and 72 °C for 45 s, followed by 72 °C for 10 min |
Reverse | 5′ GGCAGAGATGTGTTTAAGTGCTG 3′ |
Primer | Sequence | Amplification conditions |
---|---|---|
Forward | 5′ ACAATTGAATGTCTGCACAGCCACTT 3′ | 94 °C for 5 min, 35 cycles of 94 °C for 45 s, 58 °C for 45 s and 72 °C for 45 s, followed by 72 °C for 10 min |
Reverse | 5′ GGCAGAGATGTGTTTAAGTGCTG 3′ |