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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: Biochem Pharmacol. 2014 Aug 30;92(2):389–402. doi: 10.1016/j.bcp.2014.08.025

Fig. 5. Mammalian two-hybrid assay confirms that compounds affect the interaction of wild-type hPXR with SRC-1.

Fig. 5

Mammalian two-hybrid assays were performed in HepG2 cells transiently cotransfected with plasmids encoding Gal4-SRC-1 and the reporter gene pG5-luc, together with either pACT-hPXR or empty vector pACT as indicated. The cells were treated with DMSO; 5 μM rifampicin; or 5 μM, 10 μM, or 20 μM MET. Luciferase assays were performed 24 h after the compound treatment. The relative luminescence for pG5-luc was determined by normalizing firefly luciferase activity to Renilla luciferase activity. The values represent the means of five independent experiments, and the bars denote the SD (*p < 0.05; in the t-test comparisons were made between compound-treated and DMSO-treated samples in which pACT-hPXR was transfected).