Fig. 5.
Lysophospholipid:arachidonoyl-CoA acyltransferase activities in microsomes from zebrafish kidney marrow cells. Kidney marrow cell microsomes (10 µg protein) were incubated with 3 µM each of eight different acyl-CoAs and 3 µM each of six lysophospholipids, in the presence or absence of 50 µM thimerosal. Mass spectrometric analysis was performed in the negative ion mode using MRM, and results are reported as area ratio between analyte and corresponding deuterated internal standard. Results are expressed as average area ratio ± SD of at least 3 experiments. **p<0.01, ***p<0.001, calculated using repeated measures two-way ANOVA.