Fig. 2.

Mulberry leaf ethanol extract (MLEE) treatment inhibits lipid accumulation in 3T3-L1 adipocytes. At 2 days after confluence, differentiation was induced in 3T3-L1 adipocytes, and different concentrations of MLEE (0-100 µg/ml) were treated for 8 days. Eight days after differentiation and MLEE treatment, fat contents were analyzed by oil red O staining. (A-B) Representative images of Oil Red O staining. (C) Quantification of lipid accumulation based on the optical density values at 520 nm of destained Oil Red O extracted from the adipocytes. All values are mean ± SD. Mean values with different letters are significantly different at P < 0.05.