Figure 5.
The reduction in firing rate produced by glycolytic inhibition does not require TRPC channels. A, Loose-patch cell-attached recording of a TRPC3 KO SNr neuron showed a reduction in firing rate after application of IAA (1 mm) in the presence of βHB (3 mm). a, b, Traces depict cell-attached recordings of spontaneous firing before and after application of IAA in the presence of βHB (calibration: 50 pA, 200 ms). B, Glycolytic inhibition in the presence of βHB (2.5 or 3 mm) decreased the spontaneous firing rate of TRPC3 KO SNr neurons (28.6 ± 4.3 to 11.2 ± 2.8 spikes/s; n = 10; p = 6 × 10−5, Student's paired t test). C, SNr neurons lacking all seven TRPC channels are spontaneously active. Inhibition of glycolysis with IAA in the presence of βHB (2.5 mm) significantly reduces the firing rate of these neurons (28.7 ± 3.4 to 12.6 ± 1.8 spikes/s; n = 7; p = 0.001, Student's paired t test). Ctrl, Control.