Figure 2.

NG2 expression declines over time, but formerly expressing NG2-cells remain in the lesion. a–d, Level of NG2 expression at 7 (a), 14 (b), 21 (c), and 28 d (d) after injury in rat. e, A montage of the lesion in an NG2-lck-eGFP mouse 28 d after injury. Even small amounts of NG2 promoter activity are able to drive large amounts of GFP expression. f, A z-stack projection of a fiber enwrapped by a GFP+ cell at the caudal end of the lesion, 28 d after injury, from a different slice than that imaged in e. Scale bars: e, 200 μm; a–d, 100 μm; f, 25 μm. DexTR, Dextran-Texas Red