Figure 5.

Cannula tips and microinjection sites within 300 μm (25 nl injections) or 360 μm (50 nl injections) of the VLPO were considered to be hits. A, Representative image of a VLPO-containing brain slice with 488 nm Fluospheres injected bilaterally (green) to permit localization of a chronically implanted cannula tip. The white box indicates the area shown in B. B, Summary of injection locations in all successful hits for cannula experiments (red), EEG experiments (blue), and evoked potential experiments (black). In all panels, the VLPO is colored in peach. aca, Anterior commissure; 3V, third ventricle; och, optic chiasm.