Table 1.

Primer sequences for α-adrenergic receptors and GAD

Primer	Sequence (5′-3′)	PCR product (bp)
Adrenergic α1A
Outside
Forward	GAG CAA GTG ACG CTC CGT AT	348
Reverse	ACA CTG GAT TCG CAG CAC AT
Nested
Forward	ATT GTG GTG GGA TGC TTC GT	95
Reverse	ACT GTT TCC GGT GGC TTG AA
Adrenergic α1B
Outside
Forward	TTT CAT GAG GAC ACC CTC AGC AGT	303
Reverse	CAT GAA GGC GCG TTT GAA CTC CTT
Nested
Forward	CCT TGG GCA TTG TAG TCG GAA TGT	136
Reverse	TTG AAG TAG CCC AGC CAG AAC ACT
Adrenergic α1D
Outside
Forward	TCC GTA AGG CTG CTC AAG TT	176
Reverse	TAG CCC AGC CAG AAG ATG AC
Nested
Forward	CAA GAC CCT AGC CAT CGT CG	112
Reverse	GAA GAC GCC CTC TGA TGG TT
Adrenergic α2A
Outside
Forward	TGG CCA ACG AGG TTA TGG GTT ACT	210
Reverse	AGA TGA CCC ACA CGG TGA CAA TGA
Nested
Forward	TCT ATT TGG CTC TCG ACG TGC TCT	115
Reverse	GTG CGC TTC AGG TTG TAC TCG AT
Adrenergic α2B
Outside
Forward	TCT TGG CTT CCA GCA TCG GAT CTT	457
Reverse	AAG GGT GGG TTG AAT ACT GAG GCA
Nested
Forward	TGT AAT TGC CAA ACG CAG CCA CTG	162
Reverse	TGT CCA TTA GCC TCT CCG ACA GAA
Adrenergic α2C
Outside
Forward	AAG TGT GGT GTG GTG TAT ACC TGG	291
Reverse	AGC CTA TGC AGG AGG ACA AGA TGT
Nested
Forward	ATC GTG CAC CTG TGT GCC ATT AGT	139
Reverse	GGA AGG AGA TGA CAG CGG AGA TGA
GAD1
Outside
Forward	ACA ACC TTT GGC TGC ATG TGG ATG	331
Reverse	AAT CCC ACG GTG CCC TTT GCT TTC
Nested
Forward	AGT CAC CTG GAA CCC TC	128
Reverse	GCT TGT CTG GCT GGA A