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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Microbiol Methods. 2014 Sep 16;107:58–62. doi: 10.1016/j.mimet.2014.08.026

Fig. 1.

Fig. 1

Diagrammatic representation of the competitive ELISA for detection of c-di-AMP. Symbols are defined where appropriate. The first part of the assay relies on the coating of wells with CabP protein followed by a standard blocking step with BSA. A mixture of biotin-labeled and unlabeled c-di-AMP in standards or samples is then added to create the competitive condition in each well. Finally, the bound B-c-di-AMP is detected by incubation with HRP-conjugated streptavidin and followed by reaction with an OPD substrate.