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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cytokine. 2014 Sep 29;71(1):89–100. doi: 10.1016/j.cyto.2014.09.003

Figure 5. Effect of miR-146a modulation on IL-6 expression.

Figure 5

The 3′-UTR of IL-6 was cloned from PBMC samples of normal controls and sepsis patients, and subcloned into a dual-luciferase miRNA target expression vector to construct IL-6 3′-UTR reporter1 (from normal control) and reporter2 (from sepsis patient). Then, IL-6 3′-UTR reporter constructs were introduced into THP-1 cells with miR-146a. After culture, luciferase activities were analyzed to determine the association between miR-146a and the 3′UTR of IL-6 (A). miR-146a was introduced into THP-1 cells. After LPS stimulation, IL-6 release from THP-1 cells was determined by ELISA (B). THP-1 cells were incubated with different concentrations of IL-6 and 3H-thymidine incorporation was analyzed (C). P values between control and treated groups were presented.