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. Author manuscript; available in PMC: 2015 Oct 23.
Published in final edited form as: Cell. 2014 Oct 9;159(3):635–646. doi: 10.1016/j.cell.2014.09.039

Figure 1. Identification of an antibody-peptide pair that binds tightly in vivo.

Figure 1

(A–B) Schematic of the antibody-peptide labeling strategy. (B) schematic of the experiment described in (C) in which the mitochondrial targeting domain of mitoNEET (yellow box, mito) fused to mCherry and 4 tandem copies of a peptide recruits a GFP-tagged intracellular antibody to mitochondria (C) ScFv-GCN4-GFP was co-expressed with either mito-mCherry-4xGCN4peptide (lower panel) or mito-mCherry-FKBP as a control (upper panel) in U2OS cells, and cells were imaged using spinning disk confocal microscopy. Scale bars, 10 µm. See also figure S1.