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. Author manuscript; available in PMC: 2015 Dec 30.
Published in final edited form as: J Neurosci Methods. 2014 Sep 22;238:95–104. doi: 10.1016/j.jneumeth.2014.09.013

Figure 2.

Figure 2

Wnt3a induces AChR cluster elimination when DHPR and RyR1 function is inhibited. a–i, Wnt3a (30ng/ml) treated diaphragm explants with α-bungarotoxin labeled AChR clusters. a–f, Wildtype (a, d) and Mnx1null (b, e) littermate diaphragm explants treated in Wnt3a without (a, b) and with 10μM nitrendipine, an antagonist to DHPR channels (d, e; red outlined bars in c, f). Percent regionalization (c) and number (f) of AChR clusters. RyR1−/− (g) and RyR1−/−; Mnx1null (h) explants treated with Wnt3a. i, Number of AChR clusters. Asterisks show significance (p<0.005) of samples compared to control explants. Scale bar = 200 μm; yellow vertical line on the left of each panel represents the largest width of the AChR cluster region within ventral quadrant of diaphragm shown. Each condition was performed in 5 diaphragms.