Figure 6.

Intrinsic mechanisms of redistribution of AChR clusters. PKA activation by 6-Benz cAMP (a–c) narrowed the AChR cluster patterns in RyR1^null; Mnx1^null (b,c) similar to RyR1^WT; Mnx1^null (a,c) explant cultures, whereas cAMP (EPAC) activation by 8-CPT-2′-O-Me cAMP did not (d–f). g–i, Inhibition of CDK5 activity by roscovitine expanded the patterned region in RyR1^WT; Mnx1^null explants (g), to a region more similar to that of RyR1^null; Mnx1^null explants. Asterisks show significance (p<0.05) of samples to explant controls of RyR1^WT and RyR1^null. Scale bar = 200 μm; yellow vertical line represents the largest width of the AChR cluster region within ventral quadrant of diaphragm shown. Red outline in c, f, i indicate drug treatment. Each condition was performed in 5 diaphragms.