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. Author manuscript; available in PMC: 2015 Dec 30.
Published in final edited form as: J Neurosci Methods. 2014 Sep 22;238:95–104. doi: 10.1016/j.jneumeth.2014.09.013

Table 1.

Average number of AChR clusters per 100μm between the most distal α-bungarotoxin positive AChR clusters

Mnx1WT Mnx1null
Fresh-fixed Diaphragm 34+/−5 48+/−5
8 hr Diaphragm explant 37+/−6 46+/−9
Stim 5.6 mHz 31+/−9 18+/−11*
Stim 1.7mHz 39+/−11 25+/−10*
Wnt3a 40+/−10 38+/−9
Wnt3a +Nitrend. 36+/−6 8+/−2*
Wnt3a +RyR1null 22+/−3* 3+/−1*
RyR1null 43+/−7 48+/−8
Stim 5.6 mHz +RyR1null 22+/−5* 6+/−4*
NRG1 42+/−10 25+/−6*
NRG1 +RyR1null 39+/−7 28+/−4*
Agrin 43+/−10 44+/−13
Agrin +RyR1null 84+/−14* 97+/−21*
6-Benz cAMP 41+/−11 44+/−14
6-Benz cAMP +RyR1null 42+/−14 48+/−8
8-CPT-2′-O-Me cAMP 35+/−14 44+/−13
8-CPT-2′-O-Me +RyR1null 32+/−14 31+/−9
Roscovitine 43+/−10 47+/−10
Roscovitine +RyR1null 39+/−14 48+/−11

Average value from 5 diaphragm explants. Asterisks indicates p<0.005 (SEM)