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. 2014 May 16;4(1):3–7. doi: 10.1016/j.jobcr.2014.02.003

Table 1.

Primer sequence details along with PCR conditions followed.

Primer Sequence Amplification conditions
Forward
Reverse
5′ ACAATTGAATGTCTGCACAGCCACTT 3′
5′ GGCAGAGATGTGTTTAAGTGCTG 3′
94 °C for 5 min, 35 cycles of 94 °C for 45 s, 58 °C for 45 s and 72 °C for 45 s, followed by 72 °C for 10 min.