Table 1.
Primer | Sequence | Amplification conditions |
---|---|---|
Forward Reverse |
5′ ACAATTGAATGTCTGCACAGCCACTT 3′ 5′ GGCAGAGATGTGTTTAAGTGCTG 3′ |
94 °C for 5 min, 35 cycles of 94 °C for 45 s, 58 °C for 45 s and 72 °C for 45 s, followed by 72 °C for 10 min. |
Primer | Sequence | Amplification conditions |
---|---|---|
Forward Reverse |
5′ ACAATTGAATGTCTGCACAGCCACTT 3′ 5′ GGCAGAGATGTGTTTAAGTGCTG 3′ |
94 °C for 5 min, 35 cycles of 94 °C for 45 s, 58 °C for 45 s and 72 °C for 45 s, followed by 72 °C for 10 min. |