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. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: Mol Cell. 2014 Oct 8;56(3):446–452. doi: 10.1016/j.molcel.2014.09.014

Figure 2. Interaction of the ErmCL nascent chain within the ribosomal tunnel.

Figure 2

(A,B) ErmCL (teal) contacts (arrowed) with 23S rRNA nucleotides (A) U2506, U2586 and (B) A2062. (C) Toe-printing of wild-type (wt) and mutant (I6A) ErmCL translation on E. coli ribosomes with wt (U2586) or U2586A/C/G mutants, in the presence (+) or absence (−) of ERY. All reactions contained the Thr-tRNA synthetase inhibitor borrelidin which halts translation at the Thr (T11) codon in the absence of ERY-induced arrest at the Ile (I9) codon. Error bars represent one standard deviation of the mean. (D) Spacefill representation of ErmCL (teal) illustrating the steric clash with A2062 positions from PDB3OFR (Dunkle et al., 2010) (yellow) and ErmBL-SRC (PDB ID 3J5L, grey) (Arenz et al., 2014). For comparison, the A2062 position in ErmCL-SRC is shown (blue). (E) Sensing of the C3 cladinose sugar of ERY (Dunkle et al., 2010) (red) by the ErmCL nascent chain (teal). See also Figure S2–S4.