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. Author manuscript; available in PMC: 2015 Nov 7.
Published in final edited form as: J Chromatogr A. 2014 Sep 22;1367:154–160. doi: 10.1016/j.chroma.2014.09.040

Figure 2. Effect of temperature on the separation of the affinity mixture.

Figure 2

50 nM Ins* and 50 nM Ab were used in the affinity assay and 4.5 kV separations performed at the temperatures indicated on the left of the figure. The separation shown at 28 °C was performed without the heat sink on the device and the separation at 26 °C was performed with the Peltier and heat sink on the device and air blowing over the heatsink, but no active cooling was being performed. The traces are offset for clarity but are maintained to scale.