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. Author manuscript; available in PMC: 2015 Nov 7.
Published in final edited form as: J Chromatogr A. 2014 Sep 22;1367:154–160. doi: 10.1016/j.chroma.2014.09.040

Figure 5. Calibration plot of an insulin immunoassay performed at 21 °C and 6 kV.

Figure 5

Bound and free were determined by the areas of the respective peaks. Each point contains an average of 5 runs and error bars represent +/− 1 standard deviation. The data was fit with a four parameter logistic fit (black line) and a 1 nM LOD was calculated.