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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Mol Biochem Parasitol. 2014 Aug 29;196(2):90–99. doi: 10.1016/j.molbiopara.2014.08.005

Fig. 3.

Fig. 3

LmjPEM1 and LmjPEM2 are expressed throughout the life cycle of Leishmania. (A) Growth phase dependent PEMT assay was performed as described in Materials and methods. The enzymatic assay was carried out twice in duplicate and a representative experiment is shown. Standard deviations are depicted. An activity of 100% corresponds to approximately 15 pmol/mgxhr. (B) Western blot with whole cell extract derived from promastigotes harvested in mid log phase [54], 1 day (S1) or 3 day (S3) stationary growth phase, and from amastigotes (ama) in the presence of anti-LmjPEM1, anti-LmjPEM2, and anti-BIP sera [39]. Approximately 2×107 cells were loaded in each lane. The protein ladder is shown on the left.