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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Mol Biochem Parasitol. 2014 Aug 29;196(2):90–99. doi: 10.1016/j.molbiopara.2014.08.005

Fig. 7.

Fig. 7

LmjPEM1 and LmjPEM2 complement the choline auxotrophy phenotype of S. cerevisiae double null mutant scpem1Δscpem2Δ that lacks PEMT activity. Growth curves in minimal medium in the presence of 10 µM (A) or absence of choline (B). (A), (B) Open diamonds, scpem1Δscpem2Δ+U+L; open triangles, scpem1Δscpem2Δ+LmjPEM1+L; grey triangles, scpem1Δscpem2Δ+U+LmjPEM2; black triangles, scpem1Δscpem2Δ+LmjPEM1+LmjPEM2; open circles, scpem1Δscpem2Δ+ScPEM1+L; grey circles, scpem1Δscpem2Δ+U+ScPEM2; black circles, scpem1Δscpem2Δ+ScPEM1+ScPEM2. This experiment was carried out in duplicate and was repeated at least once. A typical experiment is represented.