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SDS-PAGE results for TRX-TAT-gelonin and TAT-gelonin. Lane M is a marker of the protein molecular weight standard (Invitrogen), Lane N is the purified TRX-TAT-gelonin by using the Ni-NTA chromatography, and Lane H is the final TAT-gelonin product after removal of the thioredoxin-6×His tag from TRX-TAT-gelonin and subsequent purification with heparin chromatography. (TRX-TAT-gelonin: thioredoxin-6×His tagged TAT-gelonin)
