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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: J Control Release. 2014 Sep 7;194:197–210. doi: 10.1016/j.jconrel.2014.08.030

Figure 4.

Figure 4

Elution profiles of T84.66-Hep and T84.66 from anion exchange column. While a single peak (eluted at 0 M NaCl with retention time of 2 min) was observed when T84.66 was loaded to the anion exchange column and a NaCl salt gradient was applied from 0 to 2 M (red dotted line), when the reactants of T84.66 and heparin conjugation were loaded onto anion exchange column, three major fractions labeled as Peaks 1 - 3 eluted at 0, 0.36 - 0.5 M and 0.8 –1.8 M NaCl (with retention times of 2, 28 and 50 min, respectively). (RAU: relative arbitrary unit indicating relative intensity of the absorbance at 280 nm, T84.66-Hep: T84.66-heparin conjugate)